Sperm viability in Drosophila – visualising dead and live sperm
Supervisor: Klaus Reinhardt
Background: Sperm are poor little individuals, they die relatively quick. This can be assessed by a simple assay whereby live sperm are stained green, dead sperm red and viewed under an epifluorescent microscope. Surprisingly, even in Drosophila, every lab in the world seems to use a different protocol to measure sperm viability in terms of sperm buffer used, temperature, staining time and everything else. Do you want to be the person helping to change that and developing a standardised protocol? Please get in touch.
References: start with these two from our lab:
Guo R, Henke A-L, Reinhardt K. 2020. Sperm viability varies with buffer and genotype in Drosophila melanogaster. Fly. Link
Eckel B, Guo R, Reinhardt K. 2017. More pitfalls with sperm viability staining and a viability-based stress test to characterise sperm quality. Frontiers in Ecology and Evolution. Link