Culturing a copulatory organ

Culturing an organ that serves copulation and immune function in bedbugs

Supervisor: Klaus Reinhardt, Christoph von Bredow

Background: Bedbugs have a unique organ that functions in copulation and immunity. To study these function, the organ needs to be kept in in-vitro culture. But what are the best conditions to keep the organ in? If you want to be the one who finds out – please get in touch. You will learn about cell culture, microscopy, protein analysis and various electrophoresis methods, and, if you like, methods of innate/ insect immunology.

References:

Klein, C., & Kallenborn, H. (2000). Ultrastrukturelle Untersuchungen zum Spermientransfer bei der Bettwanze Cimex lectularius, (Cimicidae, Heteroptera). Heteropteron, Köln, 8, 11-12.
Pant, U., Dhanda, V., Aragade, S. P., & Banerjee, K. (1988). Establishment of three cell lines from the embryonic tissue of Cimex hemipterus (F.)(Hemiptera: Cimicidae). In vitro cellular & developmental biology, 24(12), 1201-1203.
Pudney, M., & Lanar, D. (1977). Establishment and characterization of a cell line (BTC-32) from the triatomine bug, Triatoma infestans (Klug)(Hemiptera: Reduviidae). Annals of Tropical Medicine & Parasitology, 71(1), 109-118.