We use a number of methods to examine metabolic processes, mainly related to mitochondrial genetic variation.

  1. Time-resolved microflometry to measure the in-situ rate of ROS production simultaneoulsy with the metabolic rate of cells. See ROS.
  2. Metabolic Flux Analysis using Seahorse technology to measure the relative contributions of glycolysis and oxphos, ATP content and proton leak and maximum respiration. See ECAR.
  3. Fluorescence lifetime imaging microscopy (FLIM) with multi-photon excitation to measure metabolic rate, somewhen hopefully in vivo. See FLIM.