We use a number of methods to examine metabolic processes, mainly related to mitochondrial genetic variation.
- Time-resolved microflometry to measure the in-situ rate of ROS production simultaneoulsy with the metabolic rate of cells. See ROS.
- Metabolic Flux Analysis using Seahorse technology to measure the relative contributions of glycolysis and oxphos, ATP content and proton leak and maximum respiration. See ECAR.
- Fluorescence lifetime imaging microscopy (FLIM) with multi-photon excitation to measure metabolic rate, somewhen hopefully in vivo. See FLIM.